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Removing "c:\mb\node-pre-gyp\test\app2\lib\binding\Release\node-pre-gyp-test-app2" Gyp ERR! stack at FSReqWrap.oncomplete (fs.js:82:15) Gyp ERR! stack at C:\Program Files (x86)\nodejs\node_modules\npm\node_modules\mkdirp\index.js:48:26 Gyp ERR! stack at C:\Program Files (x86)\nodejs\node_modules\npm\node_modules\node-gyp\lib\install.js:185:19 Gyp ERR! stack at download (C:\Program Files (x86)\nodejs\node_modules\npm\node_modules\node-gyp\lib\install.js:438:22) Gyp ERR! stack at readCAFile (C:\Program Files (x86)\nodejs\node_modules\npm\node_modules\node-gyp\lib\install.js:466:15) Gyp ERR! stack at Object.fs.readFileSync (fs.js:397:15) Gyp ERR! stack at Object.fs.openSync (fs.js:549:18) Gyp ERR! stack Error: ENOENT: no such file or directory, open 'c:\mb\node-pre-gyp\test\app2\ undefined' " node-pre-gyp rebuild -C c:\mb\node-pre-gyp\test\app2 -clang=1 -msvs_version=2015 -custom_include_path=./include -toolset=cpp11 -loglevel=error" I've only been able to reproduce it with npm test which sometimes errors like on AppVeyor (sometimes not): Error: Command failed: C:\WINDOWS\system32\cmd.exe /s /c The error is intermittent, sometimes it occurs and sometimes it does not. The level of resistance in the highly susceptible wheat cultivar Bobwhite was significantly higher in transgenic plants compared to control Bobwhite and two untransformed commercial wheat cultivars, susceptible Wheaton and tolerant ND 2710. graminearum compared to control wheat plants. Transgenic wheat showed a significant reduction of disease incidence caused by F. Transformants were analyzed by Northern and Western blots to determine lactoferrin gene expression levels and were inoculated with the head blight disease fungus F. A construct containing a bovine lactoferrin cDNA was used to transform wheat using an Agrobacterium-mediated DNA transfer system to express this antimicrobial protein in transgenic wheat. Using the tools of plant genetic engineering, a broad-spectrum antimicrobial gene was tested for resistance against head blight caused by Fusarium graminearum Schwabe, a devastating disease of wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) that reduces both grain yield and quality. The development of plant gene transfer systems has allowed for the introgression of alien genes into plant genomes for novel disease control strategies, thus providing a mechanism for broadening the genetic resources available to plant breeders. Han, Jigang Lakshman, Dilip K Galvez, Leny C Mitra, Sharmila Baenziger, Peter Stephen Mitra, Amitava Transgenic expression of lactoferrin imparts enhanced resistance to head blight of wheat caused by Fusarium graminearum. 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